- Original Article
- Effect of Xanthine Oxidase Inhibitor on
Cerebral Hypoxia-Ischemia in Neonatal Rats
-
Dae-Ho Choi, Yeon-Kyun Oh, Seung-Tak Park
-
Clin Exp Pediatr. 2002;45(6):732-742. Published online June 15, 2002
-
|
Purpose : In order to evaluate the hypoxia-ischemia(H-I) induced neurotoxicity and the protective effect of xanthine oxidase(XO) inhibitor(allopurinol), cell number, cell viability, lactate dehydrogenase(LDH), protein synthesis(PS) and protein kinase C(PKC) activity were measured in cerebral neurons and astrocytes.
Methods : Cytotoxic effect was measured by in vitro assay at 12-72 hours after H-I on cerebral neurons and astrocytes derived from 7-day... |
-
-
- The Effects of Glutamate Receptor Antagonists on Cultured Cerebral Cortical Neurons of Neonatal Mouse Damaged by Oxidative Stress
-
Dae-ho Choi, Yeon-kyun Oh, Seung-taek Park
-
Clin Exp Pediatr. 1999;42(8):1096-1103. Published online August 15, 1999
-
|
Purpose : To evaluate neurotoxic effects induced by oxygen-radicals, which were generated by adding xanthine oxidase(XO) and hypoxanthine(HX), and protective effects of glutamate receptor antagonist such as MK-801 and 6-cyano-7-nitroquinoxaline(CNQX).
Methods : Dissociated cell cultures were prepared from cerebrum of neonatal mouse. Tissues were dissected and diced into small pieces in phosphate buffered saline and were incubated at 37℃. Isolated cells... |
-
-
|